DIAPH1 anticorps (Internal Region)

N° du produit ABIN488038

Détail du produit anti-DIAPH1 anticorps

Antigène: DIAPH1 (Diaphanous Homolog 1 (DIAPH1))

Épitope: Internal Region

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp DIAPH1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificité: This antibody is expected to recognize both reported isoforms (NP_005210.3 and NP_001073280.1).

Réactivité croisée (Details): Species reactivity (expected):Human.
Species reactivity (tested):Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-QTKTSKAKKDQ, from the internal region of the protein sequence according to NP_005210.3 and NP_001073280.1. Genename: DIAPH1

Information d'application

Indications d'application: Peptide ELISA: Detection Limit Dilution: 1/32000. Western blot: 0.3-1 μg/mL. Approx 140 kDa band observed in lysates of cell line NIH3T3(calculated MW of 140 kDa according to Human NP_005210.3 and 130 kDa according toMouse NP_031884.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur DIAPH1

Antigène: DIAPH1 (Diaphanous Homolog 1 (DIAPH1))

Autre désignation: DIAPH1 (DIAPH1 Produits)

Synonymes: anticorps DIAPH1, anticorps dia1, anticorps drf1, anticorps dfna1, anticorps diap1, anticorps lfhl1, anticorps DIAPH2, anticorps Diap1, anticorps D18Wsu154e, anticorps Dia1, anticorps Diaph1, anticorps Drf1, anticorps mKIAA4062, anticorps p140mDia, anticorps DFNA1, anticorps DIA1, anticorps DRF1, anticorps LFHL1, anticorps hDIA1, anticorps diaphanous related formin 1, anticorps diaphanous related formin 2, anticorps diaphanous-related formin 1, anticorps protein diaphanous homolog 1, anticorps DIAPH1, anticorps diaph1, anticorps DIAPH2, anticorps Diaph1, anticorps LOC100457442

Sujet: DIAPH1 binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity). In hearing it may play a role in the regulation of actin polymerization in hair cells.Synonyms: DIAP1, DRF1, Diaphanous homolog 1, Diaphanous-1, Diaphanous-related formin-1

ID gène: 1729

NCBI Accession: NP_005210

UniProt: O60610

Pathways: Sensory Perception of Sound