CIITA anticorps (AA 945-1130)

N° du produit ABIN4886541

Détail du produit anti-CIITA anticorps

Antigène: CIITA (Class II, Major Histocompatibility Complex, Transactivator (CIITA))

Épitope: AA 945-1130

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CIITA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for MHC class II transactivator(CIITA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for MHC class II transactivator(CIITA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: class II, major histocompatibility complex, transactivator
Protein Name: MHC class II transactivator

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human CIITA recombinant protein (Position: E945-R1130). Human CIITA shares 84.4% amino acid (aa) sequence identity with mouse CIITA.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-CIITA (ABIN4886541)

Sun, Huang, Yu, Zhang, Xu, Zhang, Li, Dong, Guo, Wang: "Autoregulatory loop between TGF-β1/miR-411-5p/SPRY4 and MAPK pathway in rhabdomyosarcoma modulates proliferation and differentiation." dans: Cell death & disease, Vol. 6, pp. e1859, (2016) (PubMed).

Détails sur CIITA

Antigène: CIITA (Class II, Major Histocompatibility Complex, Transactivator (CIITA))

Autre désignation: CIITA (CIITA Produits)

Synonymes: anticorps CIITA, anticorps C2ta, anticorps EG669998, anticorps Gm9475, anticorps C2TA, anticorps CIITAIV, anticorps MHC2TA, anticorps NLRA, anticorps Mhc2ta, anticorps class II, major histocompatibility complex, transactivator, anticorps class II major histocompatibility complex transactivator, anticorps MHC class II transactivator, anticorps class II transactivator, anticorps CIITA, anticorps LOC710761, anticorps ciita, anticorps LOC100443575, anticorps Ciita, anticorps LOC100736732

Sujet: CIITA is a human gene which is mapped to 16p13. This gene encodes a protein with an acidic transcriptional activation domain, 4 LRRs (leucine-rich repeats) and a GTP binding domain. The protein is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the "master control factor" for the expression of these genes. Also, the protein binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction. Several transcript variants encoding different isoforms have been found for this gene.

Synonyms: C2TA | CIITA | CIITA IV | MHC2TA | NLRA | P33076

ID gène: 4261

UniProt: P33076

Pathways: Cancer Immune Checkpoints