Nucleolin anticorps (pThr76)

N° du produit ABIN4916855

Détail du produit anti-Nucleolin anticorps

Antigène: Nucleolin (NCL)

Épitope: pThr76

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Nucleolin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Specificité: Reacts with Nucleolin with phoshothreonine 76 (pT76) from human and mouse

Réactivité croisée (Details): Do not cross react with phoshothreonine 84, 92 & 106 (pT76 and pT92). The detection of this P-NCL can be done during mitosis or in the centrosome during G1 phase,no signal in G2 phase.

Purification: Purified (protein A)

Immunogène: Synthetic peptide of human Nucleolin with a phoshothreonine at position 76

Information d'application

Indications d'application:

Working dilution: Optimal dilutions should be determined by the end user.
The following are guidelines only:
WB1:100 - 1:1000 IF1:50 - 1:300

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Must be reconstituted in distilled water.

Concentration: 1 mg/mL

Buffer: Tris 0,1M, glycine 0,1M

Stock: 4 °C/-20 °C

Stockage commentaire: Short term storage at+4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Date de péremption: 6 months

Détails sur Nucleolin

Antigène: Nucleolin (NCL)

Autre désignation: Nucleolin (NCL Produits)

Synonymes: anticorps C23, anticorps B530004O11Rik, anticorps D0Nds28, anticorps D1Nds28, anticorps Nucl, anticorps wu:fa12d03, anticorps zgc:152810, anticorps nucl, anticorps LOC100282923, anticorps LOC100283058, anticorps nucleolin, anticorps nucleolin S homeolog, anticorps NCL, anticorps Ncl, anticorps ncl.S, anticorps ncl, anticorps LOC100195537, anticorps LOC100282923, anticorps LOC100283058

Sujet: Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

ID gène: 4691

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization