EGFR anticorps (pTyr869) (FITC)

N° du produit ABIN492341

L’anticorps anti-EGFR Monoclonal Souris est utilisé pour la détection de EGFR dans des échantillons de Humain et Souris. Il a été validé pour IF.

Aperçu rapide pour EGFR anticorps (pTyr869) (FITC) (ABIN492341)

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EGFR est conjugé à/à la FITC

Application: Immunofluorescence (IF)

Clone: 12A3

Détail du produit anti-EGFR anticorps (FITC)

Épitope: pTyr869

Specificité: Clone 12A3 specifically recognizes EGFR phosphorylated at Tyrosine 869 and detects EGFR activation after interaction with src kinases. Antibody AM00031FC-N does not crossreact with the highly homologues pTyr877 of activated erbB2.

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse.

Attributs du produit: Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1

Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

Immunogène: Phosphopeptide conjugated to KLHAA Sequence: E K E pY H A ERemarks: Epitope: Phosphotyrosine 869

Isotype: IgG1

Information d'application

Indications d'application: Immunocytochemistry: Use at 1-10 μg/mLUse ABIN126761 or ABIN126759 for Immunoblotting and Immunoprecipitationapplications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide / PEG and Sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-80 °C

Stockage commentaire: Aiquote and freeze in liquid nitrogen. Antibody can be stored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. ThawedAliquots may be stored at 4 °C up to 3 months.

Date de péremption: 12 months

Détails sur EGFR

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Autre désignation: EGFR / ERBB1

Sujet: EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1

ID gène: 1956

UniProt: P00533

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins