DNMT3L anticorps (N-Term)

N° du produit ABIN492471

Détail du produit anti-DNMT3L anticorps

Antigène: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Épitope: AA 1-387, N-Term

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DNMT3L est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Detects ~45 kDa protein. No cross-reactivity against DNMT3a or DNMT3B.

Attributs du produit: Synonyms: DNA (cytosine-5)-methyltransferase 3-like

Purification: Protein G Chromatography.

Immunogène: Fusion protein amino acids 1-387 of Human DNMT3L (accession number BAA95556).

Clone: S117-9

Isotype: IgG1

Information d'application

Indications d'application: Western blot: 1 μg/mL1 μg/mL was sufficient for detection of DNMT3L in 10 μg of COS cell transiently transfectedwith Flag-tagged DNMT3L lysate by colorimetric immunoblot analysis using Goatanti-Mouse IgG: HRP as the secondary antibody. Immunoprecipitation: 1.0-10 μg/mLImmunofluorescence: 1.0-10 μg/mLImmunocytochemistry: 0.1-1.0 μg/mL

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 50 % Glycerol as stabilizer and 0.09 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur DNMT3L

Antigène: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Autre désignation: DNMT3L (TRDMT1 Produits)

Synonymes: anticorps DNMT3L, anticorps D6Ertd14e, anticorps ecat7, anticorps DNA methyltransferase 3 like, anticorps DNA (cytosine-5-)-methyltransferase 3-like, anticorps DNMT3L, anticorps Dnmt3l

Sujet: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue (1-6). Studies show that DNMT3L regulates the activity of DNMT3A and DNMT3B and stimulates their catalytic activities (7). DNMT3L has specifically been linked to the process of carcinogenesis, thru its role in nuclear programming (8).Synonyms: DNA (cytosine-5)-methyltransferase 3-like

ID gène: 29947

UniProt: Q9UJW3