Recombinant TNF alpha anticorps

N° du produit ABIN4949950

L’anticorps anti-TNF alpha Monoclonal Lapin est utilisé pour la détection de TNF alpha dans des échantillons de Humain. Il a été validé pour IF et IHC (p).

Aperçu rapide pour Recombinant TNF alpha anticorps (ABIN4949950)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp TNF alpha est non-conjugé

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RMTNFA-1

Détail du produit anti-TNF alpha anticorps

Purification: Protein A affinity chromatography

Immunogène: Recombinant human protein was used as the immunogen for the TNF-alpha antibody.

Isotype: IgG kappa

Information d'application

Indications d'application: Optimal dilution of the TNF-alpha antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Immunofluorescence: 5-10 μg/mL,Immunohistochemistry (FFPE): 5-10 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the TNF-alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF-alpha

Sujet: Tumor necrosis factor alpha is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons.

The primary role of TNF is in the regulation of immune cells. TNFa, being an endogenous pyrogen, is able to induce fever, apoptotic cell death, cachexia, inflammation and to inhibit tumorigenesis and viral replication and respond to sepsis via IL1 & IL6 producing cells. Dysregulation of TNF production has been implicated in a variety of human diseases including Alzheimer's disease, cancer, major depression, Psoriasis and inflammatory bowel disease (IBD). [Wiki]

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome