PAK1 anticorps
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- Antigène Voir toutes PAK1 Anticorps
- PAK1 (P21-Activated Kinase 1 (PAK1))
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Reactivité
- Humain, Rat, Souris
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp PAK1 est non-conjugé
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Application
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificité
- This detects endogenous levels of PAK1/2/3 protein.
- Réactivité croisée (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- Affinity chromatography using epitope-specific immunogen
- Pureté
- > 95 % (by SDS-PAGE)
- Top Product
- Discover our top product PAK1 Anticorps primaire
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- Indications d'application
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Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
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- Antigène
- PAK1 (P21-Activated Kinase 1 (PAK1))
- Autre désignation
- PAK1 (PAK1 Produits)
- Synonymes
- anticorps X-PAK1, anticorps XPak1, anticorps pak, anticorps pak-1, anticorps pakalpha, anticorps PAKalpha, anticorps AW045634, anticorps PAK-1, anticorps Paka, anticorps p21 protein (Cdc42/Rac)-activated kinase 1 S homeolog, anticorps p21 (RAC1) activated kinase 1, anticorps p21 protein (Cdc42/Rac)-activated kinase 1, anticorps Serine/threonine-protein kinase pak-1, anticorps pak1.S, anticorps PAK1, anticorps Pak1, anticorps pak-1
- Sujet
- Three isoforms of serine/threonine kinases, designated αPAK p68, βPAK p65 and γPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The α, β and γPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on γPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with γPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is Mek kinase, an upstream effector of Mek4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTPdependent manner with Rac1 and Cdc42, they do not interact with Rho.Synonyms: Alpha-PAK, Beta-PAK, Gamma-PAK, OPHN3, Oligophrenin-3, PAK 1, PAK 2, PAK 3, PAK alpha, PAK beta, PAK gamma, PAK-1, PAK-2, PAK-3, PAK65, Serine/threonine-protein kinase PAK 1, Serine/threonine-protein kinase PAK 2, Serine/threonine-protein kinase PAK 3, p21-activated kinase 1, p21-activated kinase 2, p21-activated kinase 3, p58, p65-PAK
- Poids moléculaire
- approx. 60 kDa
- ID gène
- 5058
- NCBI Accession
- NP_001122092
- UniProt
- Q13153
- Pathways
- Signalisation MAPK, Signalisation RTK, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis
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