AKT2 anticorps

N° du produit ABIN498587

L’anticorps anti-AKT2 Polyclonal Lapin est utilisé pour la détection de AKT2 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IF et IHC (p).

Aperçu rapide pour AKT2 anticorps (ABIN498587)

Antigène: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AKT2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-AKT2 anticorps

Specificité: This antibody detects endogenous levels of Akt2 protein.

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse, Rat.

Purification: Affinity chromatography using epitope-specific immunogen

Pureté: > 95 % (by SDS-PAGE)

Information d'application

Indications d'application: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Détails sur AKT2

Antigène: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Autre désignation: AKT2 / PKB beta

Sujet: The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBβ or RacPK-β) and Akt3 (also designated PKBγ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-I (IGF-I) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-I-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor Wortmannin. Taken together, this data strongly suggests that the protein signals downstream of the PI kinases. Akt3 is phosphorylated on a serine residue in response to insulin, and this activation is inhibited by prior activation of protein kinase C.Synonyms: Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase

Poids moléculaire: approx. 60 kDa

ID gène: 208

NCBI Accession: NP_001617

UniProt: P31751

Pathways: Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Signalisation TLR, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling