ZAP70 anticorps

N° du produit ABIN498597

Cet anticorps anti-ZAP70 Polyclonal Lapin (ABIN498597) détecte spécifiquement ZAP70 dans WB, IF et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour ZAP70 anticorps (ABIN498597)

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ZAP70 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-ZAP70 anticorps

Specificité: This antibody detects endogenous levels of ZAP-70 protein. (region surrounding Ser313)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Pureté: > 95 % (by SDS-PAGE)

Information d'application

Indications d'application: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Détails sur ZAP70

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Autre désignation: ZAP70

Sujet: The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR) which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti α and β chains, the CD3 γ, δ and ε chains and a ζ-containing homodimer or heterodimer. The disulfide-linked Ti α-β heterodimer is responsible for antigen recognition, but the short five amino acid cytoplasmic domains of Ti α and β are unlikely to be sufficient to couple to intracellular signaling pathways. In contrast, the structured features of the CD3 and ζ subunits suggest a role in signal transduction. Of these, the ζ chain, which is expressed as either a homodimer or heterodimer, has a short extracellular domain of only nine amino acids, but a larger 113 amino acid cytoplasmic domain. A tyrosine phosphoprotein, ZAP-70, has been identified that associates with ζ and undergoes tyrosine phosphorylation following TCR stimulation.Synonyms: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZAP 70, ZAP-70

Poids moléculaire: approx. 70 kDa

ID gène: 7535

NCBI Accession: NP_001070

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway