AKT2 anticorps (pSer474)
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- Antigène Voir toutes AKT2 Anticorps
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
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Épitope
- pSer474
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Reactivité
- Humain, Souris, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp AKT2 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificité
- This antibody detects endogenous levels of Akt2 protein only when phosphorylated at Ser474. This antibody does not cross-react with Akt1 and Akt3 protein when phosphorylated at the corresponding residues.
- Réactivité croisée (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- Affinity chromatography
- Pureté
- > 95 % by SDS-PAGE
- Top Product
- Discover our top product AKT2 Anticorps primaire
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- Indications d'application
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1,0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Antigène
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
- Autre désignation
- AKT2 / PKB beta (AKT2 Produits)
- Synonymes
- anticorps AKT2, anticorps pkbb, anticorps pkbbeta, anticorps pkbss, anticorps prkbb, anticorps rac-beta, anticorps v-akt2, anticorps 2410016A19Rik, anticorps AW554154, anticorps PKB, anticorps PKBbeta, anticorps HIHGHH, anticorps PKBB, anticorps PKBBETA, anticorps PRKBB, anticorps RAC-BETA, anticorps cb945, anticorps akt2-a, anticorps AKT serine/threonine kinase 2, anticorps v-akt murine thymoma viral oncogene homolog 2, anticorps thymoma viral proto-oncogene 2, anticorps v-akt murine thymoma viral oncogene homolog 2 L homeolog, anticorps AKT2, anticorps akt2, anticorps Akt2, anticorps akt2.L
- Sujet
- AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82 % identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold, overexpression or inappropriate activation of AKT has been seen in many types of cancer.Synonyms: Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase
- Poids moléculaire
- approx. 60 kDa
- ID gène
- 208
- NCBI Accession
- NP_001617
- UniProt
- P31751
- Pathways
- Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Signalisation TLR, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
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