H2AFX anticorps (pSer139)

N° du produit ABIN498792

Cet anticorps anti-H2AFX Polyclonal Lapin (ABIN498792) détecte spécifiquement H2AFX dans WB et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour H2AFX anticorps (pSer139) (ABIN498792)

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp H2AFX est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-H2AFX anticorps

Épitope: pSer139

Specificité: This antibody detects endogenous levels of p-Histone H2A.X protein only when phosphorylated at Ser139.

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Pureté: > 95 % by SDS-PAGE

Information d'application

Indications d'application: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur H2AFX

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Autre désignation: Histone H2A.x

Sujet: Histone H2A.X is a member of the Histone H2A family, which is involved in nucleosomal organization of chromatin. The Histone H2A.X gene is located in close proximity to the porphobilinogen deaminase (PBGD) gene in both mouse and human, and maps to 9 A5.2 and 11q23.3, respectively. H2A.X differs from the other members of the H2A family by the presence of a highly conserved C-terminal motif. It is rapidly phosphorylated in response to ionizing radiation and plays an important role in the recognition and repair of DNA double stranded breaks. The phosphorylated form of H2A.X, designated γ-H2A.X, forms nuclear foci at the heavy chain constant region of cells involved in class switch recombination (CSR), a region-specific DNA reaction that replaces one immunoglobulin heavy chain constant region gene with another. The phosphorylated γ-H2A.X is also thought to initiate subsequent repair factors, including Rad50, Rad51 and BRCA1.Synonyms: H2AFX, H2AX, H2a/x

Poids moléculaire: approx. 15 kDa

ID gène: 3014

NCBI Accession: NP_002096

UniProt: P16104

Pathways: Telomere Maintenance, Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus