PAK1 anticorps (pThr402, pThr423)

N° du produit ABIN498797

L’anticorps anti-PAK1 Polyclonal Lapin est utilisé pour la détection de PAK1 dans des échantillons de Humain, Rat et Souris. Il a été validé pour IHC (p).

Aperçu rapide pour PAK1 anticorps (pThr402, pThr423) (ABIN498797)

Antigène: PAK1 (P21-Activated Kinase 1 (PAK1))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PAK1 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-PAK1 anticorps

Épitope: pThr402, pThr423

Specificité: This antibody detects endogenous levels of p-PAK1/2 protein, only when phosphorylated at Thr423/402.

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Pureté: > 95 % by SDS-PAGE

Information d'application

Indications d'application: Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur PAK1

Antigène: PAK1 (P21-Activated Kinase 1 (PAK1))

Autre désignation: PAK1

Sujet: Three isoforms of Serine/Threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac 1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. The site of autophosphorylation on αPAK is Thr 423, which is in the kinase activation loop. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is MEK kinase, an upstream effector of MEK-4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac 1 and Cdc42, they do not interact with Rho.Synonyms: Alpha-PAK, Gamma-PAK, PAK 1, PAK 2, PAK alpha, PAK gamma, PAK-1, PAK-2, PAK65, Serine/threonine-protein kinase PAK 1, Serine/threonine-protein kinase PAK 2, p21-activated kinase 1, p21-activated kinase 2, p58, p65-PAK

Poids moléculaire: approx. 60 kDa

ID gène: 5058

NCBI Accession: NP_001122092

UniProt: Q13153

Pathways: Signalisation MAPK, Signalisation RTK, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis