N4BP1 anticorps

N° du produit ABIN498976

Détail du produit anti-N4BP1 anticorps

Antigène: N4BP1 (NEDD4 Binding Protein 1 (N4BP1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp N4BP1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This antibody detects endogenous levels of N4BP1 protein. (region surrounding Tyr415)

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography

Pureté: > 95 % by SDS-PAGE

Information d'application

Indications d'application: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur N4BP1

Antigène: N4BP1 (NEDD4 Binding Protein 1 (N4BP1))

Autre désignation: N4BP1 (N4BP1 Produits)

Synonymes: anticorps AI481586, anticorps C81621, anticorps RGD1305179, anticorps wu:fi03b05, anticorps zgc:66437, anticorps NEDD4 binding protein 1, anticorps Nedd4 binding protein 1, anticorps nedd4 binding protein 1, anticorps nedd4 binding protein 1 L homeolog, anticorps N4BP1, anticorps N4bp1, anticorps n4bp1, anticorps n4bp1.L

Sujet: Nedd4-binding partner-1 (N4BP1) has been identified as a protein interactor and a substrate of the homologous to E6AP C terminus (HECT) domain-containing E3 ubiquitin-protein ligase (E3), Nedd4. Here, we describe a previously unrecognized functional interaction between N4BP1 and Itch, a Nedd4 structurally related E3, which contains four WW domains, conferring substrate-binding activity. We show that N4BP1 association with the second WW domain (WW2) of Itch interferes with E3 binding to its substrates. In particular, we found that N4BP1 and p73alpha, a target of Itchmediated ubiquitin/proteasome proteolysis, share the same binding site. By competing with p73alpha for binding to the WW2 domain, N4BP1 reduces the ability of Itch to recruit and ubiquitylate p73alpha and inhibits Itch autoubiquitylation activity both in in vitro and in vivo ubiquitylation assays.Synonyms: KIAA0615, NEDD4-binding protein 1

Poids moléculaire: approx. 100 kDa

ID gène: 9683

NCBI Accession: NP_694574

UniProt: O75113