ERN1 anticorps (Intermediate Domain)

N° du produit ABIN500052

Cet anticorps anti-ERN1 est un anticorps Lapin Polyclonal détectant ERN1 dans WB et EIA. Adapté pour Humain.

Aperçu rapide pour ERN1 anticorps (Intermediate Domain) (ABIN500052)

Antigène: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ERN1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-ERN1 anticorps

Épitope: Intermediate Domain

Specificité: IRE1p antibody was raised against a 15 amino acid peptide from near the center of human IRE1p.

Purification: Affinity chromatography purified via peptide column

Immunogène: Human IRE1p (Intermediate Domain) Peptide

Isotype: IgG

Information d'application

Indications d'application: ELISA. Western Blot: 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur ERN1

Antigène: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Autre désignation: ERN1 / IRE1

Sujet: Accumulation of malfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) and the upregulation of the ER molecular chaperones GRP78 and GRP 94 (1,2). These proteins are normally bound to ER transmembrane proteins such as IRE1p and ATF6 (3,4) but ER stress causes their dissociation. This allows IRE1p, a serine-threonine protein kinase to transduce the unfolded protein signal from the ER to the nucleus. IRE1p also has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA converting it to a potent UPR transcriptional activation (5). Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER (6). Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.Synonyms: Endoplasmic reticulum-to-nucleus signaling 1, IRE1a, IRE1p, Inositol-requiring protein 1, Ire1-alpha, Serine/threonine-protein kinase/endoribonuclease IRE1

ID gène: 2081

UniProt: O75460

Pathways: ER-Nucleus Signaling, Unfolded Protein Response