HMGB1 anticorps (AA 1-200)

N° du produit ABIN5027246

Cet anticorps anti-HMGB1 est un anticorps Souris Monoclonal détectant HMGB1 dans WB, IHC et FACS. Adapté pour Humain.

Aperçu rapide pour HMGB1 anticorps (AA 1-200) (ABIN5027246)

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HMGB1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: ABM24D3

Détail du produit anti-HMGB1 anticorps

Épitope: AA 1-200

Purification: Protein G Chromatography

Immunogène: A partial length recombinant HMGB1 protein (amino acids 1- 200) was used as the immunogen for this antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: WB: 2-4 μg/mL,FACS: 0.5 μg/10^6 cells, IHC: 1 μg/mL

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % BSA, PH 7.4

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Détails sur HMGB1

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Autre désignation: HMGB1

Sujet: HMGB1 (High-mobility group protein B1) is a 25 kDa non-histone chromatin protein, secreted by immune cells through leaderless secretory pathway. HMGB1 supports transcription and also interacts with nucleosomes to loosen packed DNA and remodel the chromatin. The presence of HMGB1 in the nucleus depends on posttranslational modifications. When the protein is not acetylated, it stays in the nucleus, but hyperacetylation on lysine residues causes it to translocate into the cytosol. HMGB1 has the ability to bend DNA in order to facilitate binding of other proteins to the DNA. HMGB1 has one important role in V(D)J recombination where act as a cofactor of the RAG complex. HMGB1 stimulates a plethora of cellular responses like inflammation, attracting inflammatory cells, recruiting stem cells and promoting their proliferation. HMGB1 is highly expressed in human kidney, tonsils, adipose tissue, adrenal cortex and amniotic fluid.

Poids moléculaire: 30 kDa

ID gène: 3146

UniProt: P09429

Pathways: Signalisation p53, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome