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Arginylation (N-Term) anticorps (Biotin)

WB Hôte: Souris Monoclonal 2A4 Biotin
N° du produit ABIN5066958
  • Antigène
    Arginylation
    Épitope
    N-Term
    Hôte
    • 15
    • 1
    Souris
    Clonalité
    • 15
    • 1
    Monoclonal
    Conjugué
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Biotin
    Application
    • 15
    • 10
    • 1
    Western Blotting (WB)
    Specificité
    Specific for N-terminal arginine, next to both glutamic acid and aspartic acid. Does not detect internal arginine.
    Purification
    Protein G Purified
    Immunogène
    Synthetic N-terminal arginylated peptide conjugated to KLH
    Clone
    2A4
    Isotype
    IgG3
  • Indications d'application
    • WB (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    A 1:1000 dilution of ABIN5066958 was sufficient for detection of N-terminal Arginylation in 0.5 ug of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Conjugated antibodies should be stored at 4°C
  • Antigène
    Arginylation
    Classe de substances
    Chemical
    Sujet
    Protein arginylation is the post-translational addition of arginine to proteins by arginyltransferase ATE1. Arginylation of proteins has been found to play an essential role in physiological pathways during embryogenesis and adulthood (1). Arginylation has also been shown to regulate cell stress responses, including ER stress, cytosolic misfolded proteins, and heat stress (2).
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