7-Ketocholesterol (7-KC) anticorps (Biotin)

N° du produit ABIN5067336

Détail du produit

Antigène: 7-Ketocholesterol (7-KC)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Biotin

Application: ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)

Specificité: Specific for 7-ketocholesterol modified proteins.

Purification: Protein G Purified

Immunogène: Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).

Clone: 3F7

Isotype: IgG3

Information d'application

Indications d'application:

• WB (1:1000)
• ICC/IF (1:50)
• ELISA (1:1000)
• optimal dilutions for assays should be determined by the user.

Commentaires:

A 1:1000 dilution of ABIN5067336 was sufficient for detection of 7-Ketocholesterol in 2 μg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détail du antigène

Antigène: 7-Ketocholesterol (7-KC)

Autre désignation: 7-Ketocholesterol

Classe de substances: Hormone

Sujet: Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. 7-ketocholesterol (7-KC) is a biomarker of lipoprotein cholesterol oxidation.