NEDD8
Reactivité: Humain
WB, IHC, ELISA
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Western Blot (1:1000) Immunohistochemistry (1:50-100) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In PBS (0.09 % sodium azide)
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Fan, Bigsby, Nephew et al.: "The NEDD8 pathway is required for proteasome-mediated degradation of human estrogen receptor (ER)-alpha and essential for the antiproliferative activity of ICI 182,780 in ERalpha-positive breast ..." dans: Molecular endocrinology (Baltimore, Md.), Vol. 17, Issue 3, pp. 356-65, (2003) (PubMed).
Bohnsack, Haas: "Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer." dans: The Journal of biological chemistry, Vol. 278, Issue 29, pp. 26823-30, (2003) (PubMed).
Wu, Yamoah, Dolios, Gan-Erdene, Tan, Chen, Lee, Wei, Wilkinson, Wang, Pan: "DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1." dans: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 28882-91, (2003) (PubMed).