Immunohistochemistry (1:50-1:100) Western Blot (1:500-1:1000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In PBS, 150 mM NaCl, pH 7.4 (50 % glycerol, 0.02 % sodium azide)
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Stockage commentaire
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Connolly, Braunstein, Formenti, Schneider: "Hypoxia inhibits protein synthesis through a 4E-BP1 and elongation factor 2 kinase pathway controlled by mTOR and uncoupled in breast cancer cells." dans: Molecular and cellular biology, Vol. 26, Issue 10, pp. 3955-65, (2006) (PubMed).
Chu, Ferro: "Identification of a hydrogen peroxide-induced PP1-JNK1-Sp1 signaling pathway for gene regulation." dans: American journal of physiology. Lung cellular and molecular physiology, Vol. 291, Issue 5, pp. L983-92, (2006) (PubMed).
Kubota, Obata, Ota, Sasaki, Ito: "Rapamycin-induced translational derepression of GCN4 mRNA involves a novel mechanism for activation of the eIF2 alpha kinase GCN2." dans: The Journal of biological chemistry, Vol. 278, Issue 23, pp. 20457-60, (2003) (PubMed).