Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Fonction
Goat polyclonal antibody raised against synthetic peptide of NUMB.
Séquence
C-PFSSDLQKTF EIEL
Specificité
This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_0.03735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally.
Réactivité croisée
Chien, Humain, Souris
Immunogène
A synthetic peptide corresponding to C-terminus of human NUMB.
NUMB
Reactivité: Humain, Souris, Rat, Poulet
WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA (1:32000) Western Blot (0.03-0.1 μg/mL) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.5 mg/mL
Buffer
In Tris saline, pH 7.3 (0.5 % BSA, 0.02 % sodium azide)
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Stockage commentaire
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Holowacz, Zeng, Lassar: "Asymmetric localization of numb in the chick somite and the influence of myogenic signals." dans: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 235, Issue 3, pp. 633-45, (2006) (PubMed).
Sanada, Tsai: "G protein betagamma subunits and AGS3 control spindle orientation and asymmetric cell fate of cerebral cortical progenitors." dans: Cell, Vol. 122, Issue 1, pp. 119-31, (2005) (PubMed).