MBD3 anticorps (C-Term)
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- Antigène Voir toutes MBD3 Anticorps
- MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))
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Épitope
- AA 264-291, C-Term
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Reactivité
- Humain, Souris
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp MBD3 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
- Immunogène
- This MBD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-291 amino acids from the C-terminal region of human MBD3.
- Isotype
- Ig Fraction
- Top Product
- Discover our top product MBD3 Anticorps primaire
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- Indications d'application
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For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 2 mg/mL
- Buffer
- Supplied in PBS with 0.09 % (W/V) sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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- Antigène
- MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))
- Autre désignation
- MBD3 (MBD3 Produits)
- Synonymes
- anticorps xmbd3, anticorps MGC69548, anticorps MBD3, anticorps DKFZp459N1635, anticorps AI181826, anticorps AU019209, anticorps methyl-CpG binding domain protein 3 S homeolog, anticorps methyl-CpG binding domain protein 3, anticorps mbd3.S, anticorps mbd3, anticorps MBD3, anticorps Mbd3
- Sujet
- DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.
- Poids moléculaire
- 33 kDa
- ID gène
- 53615
- UniProt
- O95983
- Pathways
- Chromatin Binding
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