Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogène
This UHRF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-257 amino acids from the Central region of human UHRF1.
UHRF1
Reactivité: Humain
WB, IHC, DB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Restrictions
For Research Use only
Format
Liquid
Concentration
0.5 mg/mL
Buffer
Supplied in PBS with 0.09 % (W/V) sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antigène
UHRF1
(Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1))
anticorps ICBP90, anticorps Np95, anticorps RNF106, anticorps hNP95, anticorps hUHRF1, anticorps AL022808, anticorps NP95, anticorps fb97f09, anticorps wu:fb97f09, anticorps zgc:63539, anticorps UHRF1, anticorps ubiquitin like with PHD and ring finger domains 1, anticorps ubiquitin-like, containing PHD and RING finger domains, 1, anticorps ubiquitin-like with PHD and ring finger domains 1, anticorps UHRF1, anticorps Uhrf1, anticorps uhrf1
Sujet
UHRF1 is a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone acetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint.