EGFR anticorps (Internal Region)

N° du produit ABIN5539519

Cet anticorps anti-EGFR est un anticorps Chèvre Polyclonal détectant EGFR dans WB et ELISA. Adapté pour Humain.

Aperçu rapide pour EGFR anticorps (Internal Region) (ABIN5539519)

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp EGFR est non-conjugé

Application: Western Blotting (WB), ELISA

Classe de qualité: Verified

Détail du produit anti-EGFR anticorps

Épitope: Internal Region

Fonction: EGFR

Séquence: QKGSHQISLD NPD

Specificité: This antibody is expected to recognise isoform a (NP_005219.2) only.

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 170 kDa band observed in lysates of cell line HeLa (calculated MW of 134 kDa according to NP_005219.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur EGFR

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Autre désignation: EGFR

Sujet: EGFR, epidermal growth factor receptor, erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian , HGNC:3236, ERBB, ERBB1, mENA , avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, epidermal growth factor receptor, epidermal growth fac

ID gène: 1956

NCBI Accession: NP_005219

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins