CD3 epsilon anticorps (Internal Region)

N° du produit ABIN5539697

Cet anticorps Chèvre Polyclonal détecte spécifiquement CD3 epsilon dans WB et ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour CD3 epsilon anticorps (Internal Region) (ABIN5539697)

Antigène: CD3 epsilon (CD3E)

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CD3 epsilon est non-conjugé

Application: Western Blotting (WB), ELISA

Classe de qualité: Verified

Détail du produit anti-CD3 epsilon anticorps

Épitope: Internal Region

Fonction: CD3E (cytoplasmic)

Séquence: RKGQRDLYSG LNQR

Specificité: This antibody is designed to recognize the cytoplasmic domain (aa153-207) of the protein.

Réactivité croisée: Humain, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 22 kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 23.1 kDa according to NP_000724.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur CD3 epsilon

Antigène: CD3 epsilon (CD3E)

Autre désignation: CD3E

Sujet: CD3E, CD3e molecule, epsilon (CD3-TCR complex), IMD18, T3E, TCRE, CD3-epsilon, CD3e antigen, epsilon polypeptide (TiT3 complex), T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycopro

ID gène: 916, 315609

NCBI Accession: NP_000724

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway