s100a4 anticorps

N° du produit ABIN5540655

L’anticorps Souris Monoclonal anti-s100a4 a été validé pour FACS, EIA, ELISA (Capture) et ELISA (Detection). Il convient pour détecter s100a4 dans des échantillons de Humain.

Aperçu rapide pour s100a4 anticorps (ABIN5540655)

Antigène: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp s100a4 est non-conjugé

Application: Flow Cytometry (FACS), Enzyme Immunoassay (EIA), ELISA (Capture), ELISA (Detection)

Clone: NJ-4F3

Détail du produit anti-s100a4 anticorps

Specificité: Recognizes Human S100 Calcium Binding Protein A4 (S100A4). Other species not tested.

Purification: Affinity Chromatography on Protein G

Immunogène: Genetic immunisation with cDNA encoding S100A4. Selection: based on recognition of the complete native protein expressed on transfected mammalian cells.

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: 1.2 μg/10 6 cells. ELISA: 1/200-1/400. ELISA (capture): Clone NJ-4F3-D1 as detection antibody. ELISA (detection): Clone NJ-5C6-A3 as capture antibody. Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Phosphate buffered saline, pH 7.2

Stock: 4 °C,-20 °C

Stockage commentaire: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur s100a4

Antigène: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Autre désignation: s100a4

Sujet: S100 calcium binding protein A4 (S100A4) is a member of the S100 family of calcium-binding proteins that contain two Ca(2+)-binding sites including a canonical EF-hand motif. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells. S100A4 interacts with cytoskeletal proteins and enhances metastasis of several types of cancer cells. It is secreted by unknown mechanisms, thus, paracrinely stimulating a variety of cellular responses, including angiogenesis and neuronal growth (1). S100A4 has been shown to be a prognostic marker in a number of human cancers, including esophageal-squamous cancers, non-small lung cancers, primary gastric cancers, malignant melanomas, prostate cancers, bladder cancers, and pancreatic carcinomas. The universality of S100A4 expression in a variety of cancers illustrates the potential use of S100A4 as a marker for tumor metastasis and disease progression (2).

UniProt: P26447

Pathways: S100 Proteins