SPHK1 anticorps (AA 286-315)

N° du produit ABIN5552206

L’anticorps Lapin Polyclonal anti-SPHK1 a été validé pour WB, IHC (p) et EIA. Il convient pour détecter SPHK1 dans des échantillons de Humain, Souris, Rat et Primate.

Aperçu rapide pour SPHK1 anticorps (AA 286-315) (ABIN5552206)

Antigène: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reactivité: Humain, Souris, Rat, Primate

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SPHK1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-SPHK1 anticorps

Épitope: AA 286-315

Specificité: This antibody detects SPHK1 at Center.

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogène: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the central region of Human SPHK1.

Isotype: Ig Fraction

Information d'application

Indications d'application: ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur SPHK1

Antigène: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Sujet: Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.Synonyms: SPHK, SPK, SPK 1, SPK-1

Poids moléculaire: 42517 Da

ID gène: 8877, 9606

UniProt: Q9NYA1

Pathways: VEGF Signaling