RACGAP1 anticorps (C-Term)

N° du produit ABIN374167

Détail du produit anti-RACGAP1 anticorps

Antigène: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Épitope: C-Term

Reactivité: Humain, Boeuf (Vache), Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp RACGAP1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Séquence: C-GRQGNFFASP MLK

Specificité: This antibody detects RACGAP1 at C-term.

Réactivité croisée (Details): Species reactivity (expected):Canine (Dog) and Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide from the C Terminus of the protein sequence according to NP_037409.2, NP_001119576.1, NP_001119575.1. Genename: RACGAP1

Information d'application

Indications d'application: Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: 0.1-0.3 μg/mL. Approx 60 kDa band observed in Human Testis lysates(calculated MW of 71.0 kDa according to NP_037409.2). The observed molecular weightcorresponds to earlier findings in literature with different antibodies (Toure et al. , J BiolChem 1998 Mar 13,273(11): 6019-23, PMID: 9497316). Immunohistochemistry: 1 μg/mL. In paraffin embedded Human Testis, shows nuclearstaining of many cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur RACGAP1

Antigène: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Autre désignation: RACGAP1 (RACGAP1 Produits)

Synonymes: anticorps CYK4, anticorps HsCYK-4, anticorps ID-GAP, anticorps MgcRacGAP, anticorps cyk-4, anticorps id-gap, anticorps mgcracgap, anticorps RACGAP1, anticorps MGC121578, anticorps Gap, anticorps RasGAP, anticorps Rasa, anticorps GAPX, anticorps AI227039, anticorps AI327394, anticorps Band25, anticorps GTPase, anticorps gtl11, anticorps mKIAA1478, anticorps wu:fb92c08, anticorps zgc:77839, anticorps Rac GTPase activating protein 1, anticorps rac GTPase-activating protein 1, anticorps Rac GTPase activating protein 1 L homeolog, anticorps RAS p21 protein activator 1, anticorps Rac GTPase-activating protein 1, anticorps si:ch1073-416j23.1, anticorps RACGAP1, anticorps racgap1, anticorps racgap1.L, anticorps racgap1.2.L, anticorps racgap1.2, anticorps Rasa1, anticorps Racgap1, anticorps si:ch1073-416j23.1

Sujet: Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO, RAC, and CDC42. GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.Synonyms: KIAA1478, MGCRACGAP, Male germ cell RacGap, Rac GTPase-activating protein 1

Poids moléculaire: 71kDa (NP_037409).

ID gène: 29127

NCBI Accession: NP_037409

UniProt: Q9H0H5

Pathways: Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling of Hepatocyte Growth Factor Receptor