BLM anticorps (Internal Region, pThr99)

N° du produit ABIN5596805

Détail du produit anti-BLM anticorps

Antigène: BLM (Bloom Syndrome RecQ Like Helicase (BLM))

Épitope: Internal Region, pThr99

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BLM est non-conjugé

Application: Western Blotting (WB), ELISA

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with human based on 100% sequence homology. Cross-reactivity with BLM pT99 from other sources has not been determined.

Purification: Anti-BLM pT99 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Immunogène:

Immunogen: Anti-BLM pT99 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human Bloom syndrome protein.

Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-BLM pT99 antibody is useful for ELISA, Immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~159 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.11 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur BLM

Antigène: BLM (Bloom Syndrome RecQ Like Helicase (BLM))

Autre désignation: BLM (BLM Produits)

Synonymes: anticorps BS, anticorps RECQ2, anticorps RECQL2, anticorps RECQL3, anticorps blm-A, anticorps xBLM, anticorps ORF1, anticorps Bloom syndrome RecQ like helicase, anticorps Bloom syndrome RecQ like helicase S homeolog, anticorps Bloom syndrome, RecQ like helicase, anticorps BLM, anticorps blm.S, anticorps Blm

Sujet:

Synonyms: DNA helicase, RecQ-like type 2, RECQ2, RECQL3, Bloom syndrome protein

Background: BLM pT99 product is a member of the RecQ helicase protein family. The protein contains 1417 amino acids, including one ATP binding site, one DEAH box, and two putative nuclear localization signals, as well as phosphorylation sites. BLS protein is known to associate and be phosphorylated by the ATR (ataxia telangiectasia, rad3+) protein, in response to genetic stress. The Bloom's syndrome gene product is thought to play a role in the etiology of two major human health problems, each of which is a very common complication of Bloom's syndrome, namely cancer and diabetes. Anti-BLM pT99 antibody is ideal for researchers interested in Epigenetics and Nuclear Signaling or DNA Damage & Repair research.

Gene Name: BLM

Pathways: Réparation de l'ADN