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Collagen IV anticorps (Biotin)

COL4 Reactivité: Humain, Boeuf (Vache), Mammifères ELISA, WB, IHC, IP Hôte: Lapin Polyclonal Biotin
N° du produit ABIN5596836
  • Antigène Voir toutes Collagen IV (COL4) Anticorps
    Collagen IV (COL4) (Collagen, Type IV (COL4))
    Reactivité
    • 90
    • 43
    • 41
    • 23
    • 16
    • 10
    • 4
    • 2
    Humain, Boeuf (Vache), Mammifères
    Hôte
    • 59
    • 27
    • 13
    Lapin
    Clonalité
    • 71
    • 28
    Polyclonal
    Conjugué
    • 50
    • 9
    • 7
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Cet anticorp Collagen IV est conjugé à/à la Biotin
    Application
    • 61
    • 49
    • 31
    • 29
    • 27
    • 26
    • 26
    • 26
    • 20
    • 10
    • 9
    • 7
    • 7
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
    Réactivité croisée (Details)
    Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
    Purification
    Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
    Immunogène

    Immunogen: Anti-Collagen Type IV was produced by repeated immunizations with Collagen Type IV from human and bovine placenta.

    Immunogen Type: Native Protein

    Isotype
    IgG
    Top Product
    Discover our top product COL4 Anticorps primaire
  • Indications d'application

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Anti-Collagen Type IV is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

    Western Blot Dilution: 1:2,000 - 1:10,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:20,000 - 1:100,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
    Date de péremption
    12 months
  • Cohen, Hadas, Stefania, Pagoto, Ben-Dor, Kohen, Longo, Elbaz, Dekel, Gershon, Aime, Neeman: "Magnetic Resonance Imaging Reveals Distinct Roles for Tissue Transglutaminase and Factor XIII in Maternal Angiogenesis During Early Mouse Pregnancy." dans: Arteriosclerosis, thrombosis, and vascular biology, Vol. 39, Issue 8, pp. 1602-1613, (2020) (PubMed).

    Aguilar, Gardiner: "DNA Methylation Dynamics Regulate the Formation of a Regenerative Wound Epithelium during Axolotl Limb Regeneration." dans: PLoS ONE, Vol. 10, Issue 8, pp. e0134791, (2016) (PubMed).

  • Antigène
    Collagen IV (COL4) (Collagen, Type IV (COL4))
    Autre désignation
    Collagen Type IV (COL4 Produits)
    Synonymes
    anticorps collagen, type IV, anticorps Let-2
    Sujet

    Synonyms: Arresten antibody, Canstatin antibody, Collagen Of Basement Membrane Alpha 1 Chain antibody

    Background: Anti-Collagen Type IV detects collagen. Type-IV collagen is a type of collagen found primarily in the basal lamina. It is the major structural element of basal membranes. Anti-Collagen Type IV antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.

    Gene Name: COL4A4

    ID gène
    1282
    UniProt
    P02462
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