EGF anticorps (Biotin)

N° du produit ABIN5687626

L’anticorps anti-EGF Polyclonal Chèvre est utilisé pour la détection de EGF dans des échantillons de Souris. Il a été validé pour WB, ELISA et Neut.

Aperçu rapide pour EGF anticorps (Biotin) (ABIN5687626)

Antigène: EGF (Epidermal Growth Factor (EGF))

Reactivité: Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp EGF est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Détail du produit anti-EGF anticorps (Biotin)

Purification: Anti-mEGF specific antibody was purified by affinity chromatography and then biotinylated.

Immunogène: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mEGF (murine EGF).

Information d'application

Indications d'application: Neutralization:

To yield one-half maximal inhibition [ND50] of the biological activity of Murine EGF (0.1 ng/mL), a concentration of 3.0-5.0 ng/mL of this antibody is required.ELISA:

To detect mEGF by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mEGF.

Western Blot:
To detect mEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: -20 °C

Stockage commentaire: EGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Détails sur EGF

Antigène: EGF (Epidermal Growth Factor (EGF))

Autre désignation: EGF

ID gène: 13645

UniProt: P01132

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation