Western Blotting (WB), ELISA, Enzyme Immunoassay (EIA)
Purification
cytokeratin 20 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
cytokeratin 20 Antibody was raised against synthetic peptide NSRHTVNYGSDLT found at an internal region of cytokeratin 20.
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 49 kDa band observed in Human Colon and Ileum lysates (calculated MW of 48.5 kDa according to NP_061883.1). Recommended concentration: 0.01-0.03μ,g/mL. Enzyme immunoassay:This antibody was reported to work in sandwich-type ELISA as a capture antibody.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
cytokeratin 20 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Stockage commentaire
Aliquot and store at -20°C. Minimize freezing and thawing.