TNFSF13 anticorps (AA 93-233)

N° du produit ABIN5688427

Détail du produit anti-TNFSF13 anticorps

Antigène: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Épitope: AA 93-233

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TNFSF13 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purification: APRIL (monoclonal Aprily-8) antibody was purified to >=95% in SDS-PAGE.

Immunogène: APRIL (monoclonal Aprily-8) antibody was raised against recombinant human APRIL (aa. 93-233) expressed in bacteria.

Clone: Aprily-8

Isotype: IgG1

Information d'application

Indications d'application: APRIL antibody can be used to recognize human APRIL by Flow Cytometry, immunocytochemistry and Western Blot. Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with 4 % formaldehyde 5 min at RT. After a wash in PBS, samples were dehydrated by washes in 60 %, 80 %, 90 %, 100 % EtOH and xylol. Cells were then dried and embedded in paraffin. Sections were cut, mounted on slides and dried overnight at 50˚,C. Slides were then successively washed 2x 10 min in xylol, 2x 10 min in 100 % EtOH, and then treated 10 min in 100 % MeOH/ 0.6 % H202 to inhibit endogenous peroxidase. Samples were rehydrated by washes in 90 %, 80 %, 60 % EtOH and PBS. After micro-wave treatment, slides were washed 3x in PBS, blocked with IgG, and incubated for 1 hour with 5 μ,g/mL Aprily-8 or control mouse IgG (isotype control) in 1 %BSA / 1x PBS for 1 hour. After PBS washes, samples were incubated with the secondary Ab for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC. Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for human APRIL. Cells (5x105) were incubated on ice for 30 min in 50 μ,l FACS buffer (PBS, 5 % fetal calf serum, 0.02 % azide) containing 10 μ,g/mL of Aprily-8 antibody. After washing in FACS buffer, FITC-conjugated antibody to mouse IgG was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry. Method: 10 μ,g of protein was applied to the gel, and revealed with Aprily-8 (1 μ,g/mL) and HRP-coupled anti-mouse secondary antibody (1:4,000).Note: The hAPRIL construct used in this experiment is an uncleavable fusion protein between hBAFF (aa 1-132) and hAPRIL (aa 93-233). This product is for research use only.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS containing 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: APRIL antibody can be stored at 4°C, stable for one year.

Détails sur TNFSF13

Antigène: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Autre désignation: APRIL (TNFSF13 Produits)

Synonymes: anticorps APRIL, anticorps CD256, anticorps TALL-2, anticorps TALL2, anticorps TRDL-1, anticorps ZTNF2, anticorps 2310026N09Rik, anticorps April, anticorps Tall2, anticorps Trdl1, anticorps SLAN, anticorps ANP32B, anticorps april, anticorps tnf13, anticorps TNLG7B, anticorps TNF superfamily member 13, anticorps tumor necrosis factor (ligand) superfamily, member 13, anticorps acidic leucine-rich nuclear phosphoprotein 32 family member B, anticorps TNFSF13, anticorps Tnfsf13, anticorps LOC494442, anticorps tnfsf13

ID gène: 8741

UniProt: O75888

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response