EGF anticorps

N° du produit ABIN5688983

Cet anticorps anti-EGF est un anticorps Lapin Polyclonal détectant EGF dans WB, ELISA et Neut. Adapté pour Humain.

Aperçu rapide pour EGF anticorps (ABIN5688983)

Antigène: EGF (Epidermal Growth Factor (EGF))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EGF est non-conjugé

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Détail du produit anti-EGF anticorps

Purification: Anti-hEGF specific antibody was purified by affinity chromatography employing immobilized hEGF matrix.

Immunogène: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hEGF.

Information d'application

Indications d'application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hEGF (2.0 ng/mL), a concentration of 0.25 0.35 μ,g/mL of this antibody is required.

ELISA:

To detect hEGF by direct ELISA (using 100us antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Sandwich: To detect hEGF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human EGF (XP-5119Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEGF.

Western Blot:
To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: -20 °C

Stockage commentaire: EGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Détails sur EGF

Antigène: EGF (Epidermal Growth Factor (EGF))

Autre désignation: EGF

Poids moléculaire: 6.2 kDa

ID gène: 1950

UniProt: P01133

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation