To detect hLIGHT by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15-0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hLIGHT.
Sandwich:
To detect hLIGHT by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human LIGHT (XP-5215) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLIGHT.
Western Blot:
To detect hLIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLIGHT is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Restrictions
For Research Use only
Format
Lyophilized
Stock
-20 °C
Stockage commentaire
LIGHT antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Antigène
TNFSF14
(Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
LIGHT is a type II membrane protein belonging to the TNF family of membrane-anchored ligands. It is expressed in splenocytes, activated PBL, CD8+ tumor infiltrating lymphocytes, granulocytes and monocytes. LIGHT has the ability to activate NFkB, stimulate proliferation of lymphocytes, and induce apoptosis in certain human tumor cells.