PKC beta anticorps (pThr641)

N° du produit ABIN5689220

Cet anticorps anti-PKC beta Polyclonal Lapin (ABIN5689220) détecte spécifiquement PKC beta dans WB, IHC et IF. L’anticorps est réactif avec des échantillons de Humain, Rat et Souris.

Aperçu rapide pour PKC beta anticorps (pThr641) (ABIN5689220)

Antigène: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PKC beta est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-PKC beta anticorps

Épitope: pThr641

Specificité: This antibody detects endogenous levels of PKCβ, only when phosphorylated at threonine 641.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogène: PKCbeta (Phospho-Thr641) antibody was raised against a peptide sequence around phosphorylation site of threonine 641 (E-L-T (p) -P-T) derived from Human PKCβ,.

Information d'application

Indications d'application: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store antibody at -20°C for up to one year.

Détails sur PKC beta

Antigène: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Autre désignation: PKCbeta

Sujet: Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-κ,-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-κ,-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.

Poids moléculaire: 82 kDa

ID gène: 5579

NCBI Accession: NP_002729

UniProt: P05771

Pathways: Signalisation WNT, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling