ME2 antibody is human specific. At least two isoforms of ME2 are known to exist, this antibody will detect both isoforms. ME2 antibody is predicted not to cross-react with ME1.
Purification
ME2 antibody is affinity chromatography purified via peptide column.
Immunogène
ME2 antibody was raised against a 17 amino acid peptide near the center of human ME2.
The immunogen is located within amino acids 340 - 390 of ME2.
NAD-ME
Reactivité: Humain
WB, ELISA, IHC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ME2 antibody can be used for detection of ME2 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 5 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
ME2 Antibody is supplied in PBS containing 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
ME2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
ME2 is a homotetrameric, mitochondrial NAD-dependent malic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate (1). It is related to malic enzyme 1 (ME1), a cytoplasmic NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis (2). The expression of both malic enzymes is reciprocally regulated by p53, this regulation has been shown to modulate metabolism and senescence (3). Certain single-nucleotide polymorphism haplotypes of the ME2 gene have been shown to increase the risk for idiopathic generalized epilepsy (4).