TNFSF13 anticorps (AA 105-250)

N° du produit ABIN5690647

Détail du produit anti-TNFSF13 anticorps

Antigène: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Épitope: AA 105-250

Reactivité: Humain, Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp TNFSF13 est non-conjugé

Application: ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purification: APRIL (monoclonal Sacha-2) antibody was purified from concentrated hybridoma tissue culture supernatant. APRIL (monoclonal Sacha-2) antibody was purified to 95% in SDS-PAGE.

Immunogène: APRIL (monoclonal Sacha-2) antibody was raised against recombinant human APRIL (aa. 105-250).

Clone: Sacha-2

Isotype: IgG2a

Information d'application

Indications d'application: APRIL antibody can be used to recognize human and mouse APRIL by ELISA (capture), Flow Cytometry, and Immunocytochemistry. Method: HEK-293T cells expressing surface membrane associated human APRIL (aa 92-233) or a mock control were injected in chicken liver and frozen in OCT. 8 μ,m sections of injected frozen liver were fixed in acetone, washed in PBS and incubated with Sacha-2 at 10 μ,g/mL for 1hr after blocking in PBS/1 % BSA. Human APRIL was revealed with 3-amino-9-Ethyl-carbazole (AEC) following 45 min. incubation with goat anti-rat HRP. Method: HEK 293T  cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mouse APRIL (thick line). Cells were incubated on ice for 30 min in 50 μ,l FACS buffer (PBS, 5 % Fetal calf serum, 0.02 % sodium azide) containing 1 μ,g/mL of Sacha-2 antibody.  After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry. This product is for research use only.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS containing 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: APRIL antibody can be stored at 4°C, stable for one year.

Détails sur TNFSF13

Antigène: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Autre désignation: APRIL (TNFSF13 Produits)

Synonymes: anticorps APRIL, anticorps CD256, anticorps TALL-2, anticorps TALL2, anticorps TRDL-1, anticorps ZTNF2, anticorps 2310026N09Rik, anticorps April, anticorps Tall2, anticorps Trdl1, anticorps SLAN, anticorps ANP32B, anticorps april, anticorps tnf13, anticorps TNLG7B, anticorps TNF superfamily member 13, anticorps tumor necrosis factor (ligand) superfamily, member 13, anticorps acidic leucine-rich nuclear phosphoprotein 32 family member B, anticorps TNFSF13, anticorps Tnfsf13, anticorps LOC494442, anticorps tnfsf13

ID gène: 8741, 9370

UniProt: Q15848

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response