p21 anticorps (C-Term)

N° du produit ABIN5691109

L’anticorps Souris Monoclonal anti-p21 a été validé pour WB, ELISA, FACS, IHC (p), ICC et IHC (fro). Il convient pour détecter p21 dans des échantillons de Humain et Singe.

Aperçu rapide pour p21 anticorps (C-Term) (ABIN5691109)

Antigène: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reactivité: Humain, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp p21 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: WA1

Détail du produit anti-p21 anticorps

Épitope: C-Term

Specificité: The antibody WA1 is directed against human p21/WAF1, but also recognizes this protein in non-human primates . Reports suggest that this antibody recognizes an epitope within the carboxy-terminal region of p21/WAF1.

Immunogène: WA1 is a mouse IgG1 monoclonal antibody derived from the fusion of SP2 mouse myeloma cells with splenocytes from a BALB/c mouse immunized with human p21 protein.

Isotype: IgG1

Information d'application

Indications d'application: The WA1 antibody is suitable for the detection of p21/WAF1 by flow cytometry, immunocytochemistry, ELISA and western blotting. The antibody is also suitable for the detection of p21/WAF1 by immunohistochemistry of frozen and paraffin embedded tissues. Paraffin embedded tissues require pre-treatment prior to staining with the WA1 antibody. Antigen retrieval by heat treatment using sodium citrate buffer is suggested for this purpose. Optimal antibody dilutions for the different applications should be determined by titration.

Restrictions: For Research Use only

Stockage

Buffer: Each vial contains 100 μg 1 mg/mL purified monoclonal antibody in PBS containing 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C, or in small aliquots at -20°C.

Détails sur p21

Antigène: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Autre désignation: p21/WAF1/ cip1/Cyclin-dependent kinase inhibitor 1

Sujet: P21/WAF1, also known as cip1 and cyclin-dependent kinase interacting protein 1, is a 21 kDa protein that belongs to the CDI family of cyclin-dependent kinase inhibitors. In humans p21/WAF1 is encoded by the CDKN1A gene, the transcription of which is regulated by P53, for example in the case of DNA damage. The p21/WAF1 protein exists within the nucleus as a complex with cyclin, cyclin-dependent kinase (cdk) and proliferating cell nuclear antigen (PCNA). It functions as a potent inhibitor of cyclin-dependent kinases and plays a critical role in regulating cell growth and the cellular response to DNA damage. Studies show that the amino-terminal domain of p21/WAF1 binds to cyclin/cdk complexes and inhibits their activity, preventing cell cycle progression at the G1/S phase. In addition, p21/WAF1 can also bind through its carboxy-terminal domain to PCNA, inhibiting the activity of PCNA and, as a result, DNA replication. Expression of p21/WAF1 is tightly regulated at the transcriptional level by the tumour suppressor protein p53. It is reported to be upregulated in response to a variety of cellular stress stimuli. Loss of p21 expression has been associated with poor prognosis in several carcinomas including gastric carcinoma, non-small cell lung carcinoma, and thyroid carcinoma.

UniProt: P38936

Pathways: Signalisation p53, Signalisation PI3K-Akt, Cycle Cellulaire, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy