WFDC2 anticorps (Internal Region)

N° du produit ABIN569663

Détail du produit anti-WFDC2 anticorps

Antigène: WFDC2 (WAP Four-Disulfide Core Domain 2 (WFDC2))

Épitope: AA 29-43, Internal Region

Reactivité: Humain, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp WFDC2 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Séquence: GAEKTGVCPE LQADQ

Specificité: Recognizes WFDC2 / WAP5 (29-43).

Réactivité croisée (Details): Species reactivity (expected):Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide with sequence from the internal region of the protein sequence according to NP_006094.3. Genename: WFDC2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur WFDC2

Antigène: WFDC2 (WAP Four-Disulfide Core Domain 2 (WFDC2))

Autre désignation: WFDC2 / WAP5 (WFDC2 Produits)

Synonymes: anticorps WFDC3, anticorps WFDC2, anticorps si:ch211-153a8.3, anticorps EDDM4, anticorps HE4, anticorps WAP5, anticorps dJ461P17.6, anticorps 1600023A02Rik, anticorps re4, anticorps CE4, anticorps BE-20, anticorps WAP four-disulfide core domain 2, anticorps WFDC2, anticorps wfdc2, anticorps Wfdc2

Sujet: The whey acidic protein (WAP) domain is a conserved motif, containing eight cysteines found in a characteristic 4-disulphide core arrangement, that is present in a number of otherwise unrelated proteins. One of these proteins, which contains two WAP domains, is HE4 (also known as WFDC2), originally described as an epididymis specific protein but more recently suggested to be a putative serum tumour marker for ovarian cancer and a presumptive role in natural immunity. The HE4 protein expression is not only confined to epidydimis but is expressed in a number of normal human tissues out side the reproductive system, including regions of the respiratory tract and nasopharynx and in a subset of lung tumor cell lines. HE4 gene expression was highest in normal human trachea and salivary gland, and to a lesser extent, lung, prostate, pituitary gland, thyroid, and kidney. Highest level of expression of ESPE4 was observed in adenocarcinomas of the lung, and occasional breast, transitional cell and pancreatic carcinomas. The WFDC2 gene under goes extensive splicing in malignant tissues that give rise to five WAP domain containing iso forms. WFDC2 is expressed in some epithelial cells of the upper airways as well as in mucous cells and ducts of submucosal glands. No staining was seen in peripheral lung. Intense staining is found in major salivary glands and in minor glands of the nose, sinuses, posterior tongue and tonsil. Studies with the related protein Secretory Leukocyte Protease Inhibitor (SLPI) show that although both proteins are expressed in similar tissues, the precise cellular localization differs. Significant increases in expression and localization of WFDC2 are seen in patients with Cystic Fibrosis. ESPE2 protien is a 124 amino acids (15 kDa) protein. ESPE4 protein has sequence homology to extracellular proteinase inhibitor and is localized on human chromosome 20. The ESPE4 protein is a secreted glycoprotein (Asn-44) that is over expressed in serous and endometrioid ovarian carcinomas. There are at least 5 named variants (HE4-V1 to HE4-V5) expressed as a result of alternate splicing and they serve as endopeptidase inhibitors.Synonyms: Epididymal secretory protein E4, HE4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, WAP four-disulfide core domain protein 2

ID gène: 10406

NCBI Accession: NP_006094

UniProt: Q14508