Histone H4 anticorps (acLys16)

N° du produit ABIN5706796

Détail du produit anti-Histone H4 anticorps

Antigène: Histone H4

Épitope: acLys16

Reactivité: Humain, Souris, Rat, Poulet, Xenopus laevis, Drosophila melanogaster, C. elegans, Plantes

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone H4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)

Attributs du produit:

Synonyms: rabbit anti-Histone H4 Ac Lys16 antibody, H4K16ac, HIST2H4B, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4

Background: Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, acetylation of H4 Lys16 (H4 K16Ac) plays a role in transcriptional activation and DNA repair. The protein MOF (MYST1) is the primary enzyme to acetylate K16, along with Gcn5, Esal, ATF2, and Sas2. Depletion of MOF reduces K16 acetylation, causing cell cycle arrests, chromosomal aberrations, and a poor DNA damage response. Anti-Histone H4 are ideal for researchers interested in Epigenetics, HA Epitope Tags, Chromatin Modifiers, Chromatin Research, Histones and Modified Histones research.

Gene Name: HIST4H4

Purification: Anti-Histone H4 [ac Lys16] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H4. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with most mammal species. Cross-reactivity with Histone H4 from other sources has not been determined.

Immunogène:

Immunogen: Histone H4 [ac Lys16] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic aceylated peptide surrounding Lysine 16 of human Histone H4.

Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:100

Application Note: Anti-Histone H4 [ac Lys16] antibody is useful for Western Blot, Dot Blot, Immunofluorescence, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~13 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

ChIP Dilution: not recommended

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 1:100

Commentaires:

Dot Blot 1:10000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Date de péremption: 12 months

Détails sur Histone H4

Antigène: Histone H4

Abstract: Histone H4 Produits

Synonymes: anticorps hist1h4l, anticorps H1ft, anticorps Hist4, anticorps Hist4h4, anticorps H4, anticorps X04652, anticorps FO108, anticorps H4/n, anticorps H4F2, anticorps H4FN, anticorps HIST2H4, anticorps H4C13, anticorps H4C7, anticorps F8N16.2, anticorps F8N16_2, anticorps histone H4, anticorps BcDNA:RH52884, anticorps CG3379, anticorps Dmel\CG3379, anticorps H4r, anticorps His4-88CD, anticorps His4R, anticorps histone cluster 1, H4a L homeolog, anticorps histone cluster 1 H4 family member B, anticorps histone cluster 2, H4, anticorps histone cluster 2 H4 family member a, anticorps histone H4, anticorps Histone H4 replacement, anticorps Histone H4, anticorps hist1h4a.L, anticorps Hist1h4b, anticorps Hist2h4, anticorps HIST2H4A, anticorps H4, anticorps H4C14, anticorps HIS4, anticorps HIST2H4, anticorps His4r, anticorps his-38

ID gène: 121504

NCBI Accession: NP_001029249

UniProt: P62805