p21 anticorps

N° du produit ABIN5707847

Cet anticorps Lapin Monoclonal détecte spécifiquement p21 dans IHC (p). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour p21 anticorps (ABIN5707847)

Antigène: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp p21 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CIP1-2275R

Détail du produit anti-p21 anticorps

Aucune reactivité croisée: Souris, Rat (Rattus)

Purification: Purified

Pureté: Protein A affinity chromatography

Immunogène: Recombinant full-length human protein was used as the immunogen for the recombinant CDNK1A antibody.

Isotype: IgG kappa

Information d'application

Indications d'application: Optimal dilution of the recombinant CDNK1A antibody to be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Stockage

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the recombinant CDNK1A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur p21

Antigène: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Autre désignation: p21 / CDKN1A

Sujet: This mAb recognizes a 21  kDa protein, identified as the p21WAF1 tumor suppressor protein, expressed by the CDNK1A gene. This mAb is highly specific to CDNK1A/p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk's and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of CDNK1A expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).

Pathways: Signalisation p53, Signalisation PI3K-Akt, Cycle Cellulaire, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy