N/H/K-Ras anticorps

N° du produit ABIN5956741

Détail du produit

Antigène: N/H/K-Ras

Reactivité: Souris, Rat, Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: NRAS,HRAS1,GTPase NRas,Transforming protein N-Ras,HRAS,HRAS1,GTPase HRas,H-Ras-1,Ha-Ras,Transforming protein p21,c-H-ras,p21ras,KRAS,KRAS2,RASK2,GTPase KRas,K-Ras 2,Ki-Ras,c-K-ras,c-Ki-ras

Purification: Affinity purification

Immunogène: Synthesized peptide derived from the N-terminal region of human N/H/K-Ras

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:20000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 0.5 % BSA and 50 % glycerol, pH 7.4

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze / thaw cycles.

Détail du antigène

Antigène: N/H/K-Ras

Sujet: NRAS an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. HRAS belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. KRAS, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.

Poids moléculaire: 21kDa

ID gène: 3265, 3845, 4893