V5 Epitope Tag anticorps (Biotin)

N° du produit ABIN602522

Détail du produit anti-V5 Epitope Tag anticorps (Biotin)

Antigène: V5 Epitope Tag

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp V5 Epitope Tag est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC)

Séquence: GKPIPNPLLG LDST

Specificité: Recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is, gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined.

Purification: Protein G purified

Immunogène: Paramyxovirus SV5, Simian-Virus 5.

Type of Immunogen: Virus

Isotype: IgG2a

Information d'application

Indications d'application: Approved: ELISA, IHC, IHC-Fr, WB (1:1000 - 1:5000)

Usage: Western Blot:1:1000-1:5000. ELISA. Immunohistochemistry (frozen). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: IgG conjugated to Biotin. Supplied as a liquid, 0.1 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Aliquot to Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Détails sur V5 Epitope Tag

Antigène: V5 Epitope Tag

Autre désignation: V5 Tag (V5 Epitope Tag Produits)

Classe de substances: Tag