TGF-beta anticorps

N° du produit ABIN604857

Détail du produit anti-TGF-beta anticorps

Antigène: TGF-beta (TGFb)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TGF-beta est non-conjugé

Application: Western Blotting (WB), Neutralization (Neut), ELISA, Functional Studies (Func), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marque: IHC-plus™

Specificité: Recognizes both human platelet-derived and recombinant TGF-beta.

Purification: Protein G purified

Immunogène: TGF-b from human platelets.

Type of Immunogen: Purified protein

Clone: TB21

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: ELISA, Func, IHC, IHC-Fr, IHC-P (10 μg/mL), Neut, WB

Usage: Suitable for use in Western Blot, ELISA, Neutralization and Immunohistochemistry. Neutralization: Neutralizes TGF-beta activity in vitro in an inhibition assay of CCL/64 cell growth and the growth promoting action of TGF-beta in the NRK-49F colony forming assay. The effect of microinjection of this antibody into one blastomere of two cell stage Xenopus embryos indicated that it was able to neutralize effectively the bioactivity of TGF-beta in vivo. Immunohistochemistry (formalin-fixed, paraffin-embedded, frozen): 1:1000. Has been used to demonstrate TGF-beta in ovine ovarian tissue and human breast carcinoma. As a consequence of the intense staining of the erythrocytes it is possible to locate a single cell within the ovarian stroma making it useful in locating very fine capillary networks within tissue. Western Blot: 5-20 ng/mL will allow visualization of 100 ng/lane of TGF-beta. Recognizes the dimeric (25kD) and monomeric (12.5kD) forms of TGF-beta under both non-reducing and reducing conditions respectively.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.0.

Conseil sur la manipulation: avoid freeze thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Références pour anticorps anti-TGF-beta (ABIN604857)

Baumeier, Escher, Aleshcheva, Pietsch, Schultheiss: "Plasminogen activator inhibitor-1 reduces cardiac fibrosis and promotes M2 macrophage polarization in inflammatory cardiomyopathy." dans: Basic research in cardiology, Vol. 116, Issue 1, pp. 1, (2021) (PubMed).

Détails sur TGF-beta

Antigène: TGF-beta (TGFb)

Autre désignation: TGF-beta (TGFb Produits)

Synonymes: anticorps CED, anticorps DPD1, anticorps LAP, anticorps TGFB, anticorps TGFbeta, anticorps TGF-beta, anticorps TGF-BETA-1, anticorps TGF-beta1, anticorps TGFbeta1, anticorps Tgfb, anticorps Tgfb-1, anticorps transforming growth factor beta 1, anticorps transforming growth factor beta-1, anticorps transforming growth factor, beta 1, anticorps TGFB1, anticorps Tgfb1

Pathways: EGFR Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus