HLA-DRA anticorps (CF®640R)

N° du produit ABIN6153457

L’anticorps Souris Monoclonal anti-HLA-DRA a été validé pour FACS et IF. Il convient pour détecter HLA-DRA dans des échantillons de Humain et Singe.

Aperçu rapide pour HLA-DRA anticorps (CF®640R) (ABIN6153457)

Antigène: HLA-DRA (HLA Class II DR alpha (HLA-DRA))

Reactivité: Humain, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HLA-DRA est conjugé à/à la CF®640R

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: IPO-10

Détail du produit anti-HLA-DRA anticorps (CF®640R)

Fonction: Mouse Monoclonal anti-MHC-II (HLA-DRA) (IPO-10), CF640R Conjugate

Attributs du produit: MAb IPO-10 defines the antigen, which appears on B cell progenitors following HLA-DR and preceding CD10, CD19, CD22, CD37 and cym. It is expressed on resting B cells and than reappears and persists in cytoplasm and on cell surface until cytoplasmic Ig appears. It is a useful antibody for diagnostics of neoplasms of B cell origins. It reacts with human B cell lines Daudi, Raji, Namalva, EB-3, RPMI-8226 (50 % of cells). The MAb does not label T cell lines, blood granulocytes, thymocytes or bone marrow stromal fibroblasts. No significant changes are detected after PHA or ConA stimulation while LPS and PWM stimulated cultures after 18-48h show decreased number of antigen-positive cells but in final terms of cultivation antigen is expressed again. This MAb labels B cell leukemias and some lymphomas. Hairy cell leukemia strongly reacts and 70 % of B cell CLL and some B-NHL were also positive. IPO-10 reacts with AMML cells and in a majority of Hodgkin's disease cases a significant percentage of affected lymph node cells were detected. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Spleen cells of a patient with hairy cell leukemia (Daudi cells)

Isotype: IgG3 kappa

Information d'application

Indications d'application: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

Ramos, Daudi or HuT78 cells. Tonsil or lymph node

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur HLA-DRA

Antigène: HLA-DRA (HLA Class II DR alpha (HLA-DRA))

Autre désignation: MHC-II (HLA-DRA)

Poids moléculaire: 36 kDa

ID gène: 3122, 520048

UniProt: P01903

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Human Leukocyte Antigen (HLA) in Adaptive Immune Response