UBE2F anticorps (N-Term)
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- Antigène Voir toutes UBE2F Anticorps
- UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))
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Épitope
- N-Term
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Reactivité
- Humain, Souris, Rat, Boeuf (Vache), Chien, Porc
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp UBE2F est non-conjugé
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Séquence
- SKLKRDDGLK GSRT-C
- Specificité
- Recogfnizes NEDD8 conjugating enzyme (N Terminus).
- Réactivité croisée (Details)
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Species reactivity (expected):Human, Mouse, Canine (Dog), Pig, Cow.
Species reactivity (tested):Rat. - Purification
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
- Immunogène
- Peptide with sequence from the N Terminus of the protein sequence according to NP_542409.1. Genename: UBE2F
- Top Product
- Discover our top product UBE2F Anticorps primaire
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Antigène
- UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))
- Autre désignation
- NCE2 (UBE2F Produits)
- Synonymes
- anticorps NCE2, anticorps 2510010F15Rik, anticorps AI851109, anticorps RGD1307608, anticorps zgc:77005, anticorps ubiquitin conjugating enzyme E2 F (putative), anticorps NEDD8-conjugating enzyme UBE2F, anticorps ubiquitin-conjugating enzyme E2F (putative), anticorps ubiquitin conjugating enzyme E2F (putative) S homeolog, anticorps UBE2F, anticorps Tsp_01616, anticorps ube2f, anticorps ube2f.S, anticorps Ube2f
- Sujet
- Ubiquitin is covalently attached to target proteins by a multienzymatic system consisting of E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin-ligating) enzymes. NEDD8, a ubiquitin-like protein, is conjugated to proteins in a manner analogous to ubiquitinylation. beta-amyloid precursor protein-binding protein-1 (APPBP1) can bind to NEDD8 in rabbit reticulocyte lysates. However, since APPBP1 shows similarity to only the N-terminal domain of an E1 enzyme, it must interact with a protein showing similarity to the C-terminal region of E1s. By searching sequence databases, a cDNAs encoding UBA3 was identified as the human homolog of yeast Uba3. The predicted 442-amino acid UBA3 protein shares 43 % sequence identity with yeast Uba3. In vitro, UBA3 formed a complex with APPBP1 and a thioester linkage with NEDD8. APPBP1/UBA3 complex may function as an E1-like enzyme for the activation of NEDD8.Synonyms: NEDD8-conjugating enzyme 2, UBE2F, Ubiquitin-conjugating enzyme E2 F
- ID gène
- 140739
- NCBI Accession
- NP_542409
- UniProt
- Q969M7
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