IL-1 beta anticorps

N° du produit ABIN616079

L’anticorps anti-IL-1 beta Polyclonal Lapin est utilisé pour la détection de IL-1 beta dans des échantillons de Souris et Rat. Il a été validé pour WB, IP, IF, IHC (p), Neut, EIA, IHC (fro) et RIA.

Aperçu rapide pour IL-1 beta anticorps (ABIN616079)

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-1 beta est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Détail du produit anti-IL-1 beta anticorps

Specificité: This antibody is primarily directed against mature, 17,000 MW Mouse IL1-beta and is useful in determining its presence in various assays. The antibody does not recognize Human IL1-beta or Mouse IL1-alpha. In ELISA formats and other immunoreactive assays, reactivity occurs with Rat IL1-beta. This antibody will recognize 10 % of the non-denatured (native) precursor 31,000 MW Mouse IL1-beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in Immunoblot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL1-beta precursor molecule. Denatured 31,000 precursor IL1-beta will be recognized by this antibody.

Purification: DEAE Fractionation

Immunogène: Recombinant Mouse IL-1 beta produced in E. coli Genename: IL1b Remarks: The MW of the recombinant Mouse IL-1 beta is 17 kDa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.1 mL of deionized water (or equivalent)

Concentration: 1.0 mg/mL (UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, None, None

Agent conservateur: Azide free

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C or -20 °C. Following reconstitution store at -20 °C.

Détails sur IL-1 beta

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Autre désignation: Interleukin-1 beta / IL-1B

Sujet: IL-1 beta (also known as Interleukin-1 beta, IL1-beta and catabolin) is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1beta is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.Synonyms: Catabolin, IL-1 beta, IL1 beta, IL1B, IL1F2

ID gène: 16176

NCBI Accession: NP_032387

UniProt: P10749

Pathways: Signalisation NF-kappaB, Interferon-gamma Pathway, Signalisation TLR, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome