Actin, Muscle Specific anticorps (Biotin)

N° du produit ABIN6162603

Détail du produit anti-Actin, Muscle Specific anticorps (Biotin)

Antigène: Actin, Muscle Specific

Reactivité: Humain, Lapin, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Actin, Muscle Specific est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)

Fonction: Mouse Monoclonal anti-Muscle-Specific-Actin (MSA/953), Biotin Conjugate

Attributs du produit: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Synthetic peptide common among human muscle actins

Clone: MSA-953

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1.0 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

Muscle or sarcoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur Actin, Muscle Specific

Antigène: Actin, Muscle Specific

Autre désignation: Muscle-Specific-Actin (Actin, Muscle Specific Produits)

Sujet: ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1

Poids moléculaire: Multiple

ID gène: 58, 59, 70

UniProt: P62736