HSP27 anticorps

N° du produit ABIN6164168

Cet anticorps anti-HSP27 est un anticorps Souris Monoclonal détectant HSP27 dans WB, IF, FACS, IHC (f) et ISt. Adapté pour Humain, Rat, Souris, Mouton, Chimpanzé, Poulet et Singe.

Aperçu rapide pour HSP27 anticorps (ABIN6164168)

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivité: Humain, Rat, Souris, Mouton, Chimpanzé, Poulet, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HSP27 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt)

Clone: G3-1

Détail du produit anti-HSP27 anticorps

Fonction: Mouse Monoclonal anti-HSP27 (G3.1), Purified, BSA-free

Attributs du produit: This MAb reacts specifically with heat shock protein HSP27 in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50 % homology with low molecular weight Drosophila HSPs and mammalian α-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore HSP27 may be useful in classifying various hormone sensitive tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogène: Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.25-0.5 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Agent conservateur: Azide free

Détails sur HSP27

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Autre désignation: HSP27

Poids moléculaire: 27 kDa

ID gène: 3315, 520973

UniProt: P04792

Pathways: Signalisation MAPK, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling