ICAM1 anticorps (CF®405S)
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- Antigène Voir toutes ICAM1 Anticorps
- ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp ICAM1 est conjugé à/à la CF®405S
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Fonction
- Mouse Monoclonal anti-CD54 (1H4-or-Wehi-CAM-1-or-W-CAM-1), CF405S Conjugate
- Attributs du produit
- This antibody recognizes an 85-115 kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1) (Workshop IV). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. W-CAM-1 MAb blocks aggregation of cell lines mediated by the ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and also aggregation of mixed T- and B-cell blasts. It inhibits T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T-cell mediated B-cell activation) is significantly inhibited. This MAb blocks elements of both effector arms of immune system (cytotoxic cell function and Ig production). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogène
- Raji Burkitt lymphoma cells
- Clone
- 1H4 or Wehi-CAM-1 or W-CAM-1
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product ICAM1 Anticorps primaire
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- Indications d'application
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Immunohistology formalin-fixed 2-4 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Functional Studies order Ab without BSA & azide)
- Optimal dilution for a specific application should be determined by user
- Commentaires
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Raji cells, MOLT 4, HeLa or SW480 cells. Human tonsil.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Protect from light
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- Antigène
- ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
- Autre désignation
- CD54 (ICAM1 Produits)
- Synonymes
- anticorps BB2, anticorps CD54, anticorps P3.58, anticorps Icam-1, anticorps Ly-47, anticorps MALA-2, anticorps ICAM, anticorps ICAM-1, anticorps ICAM1, anticorps intercellular adhesion molecule 1, anticorps intercellular adhesion molecule-1, anticorps ICAM1, anticorps Icam1, anticorps ICAM-1
- Classe de substances
- Viral Protein
- Sujet
- Intestinal mucin-3A, intestinal mucin-like, MUC3, MUC3A, MUC3B, mucin 3, intestinal, mucin 3A, cell surface associated, mucin 3A, intestinal, mucin 3B, cell surface associated
- Poids moléculaire
- 85-115 kDa
- ID gène
- 3383, 643447
- UniProt
- P05362
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling
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